Western Blot:Article Title: A mitotic CDK5-PP4 phospho-signaling cascade primes 53BP1 for DNA repair in G1
Article Snippet: .. List of antibodies used in this study Target Manufacturer Catalogue number Host Dilution or amount used Application p35/25 Cell Signaling Technology 2680 Rabbit 1:500 Immunoblotting 53BP1 Santa Cruz sc-22760 Rabbit 5ug per mg lysate Immunoprecipitation 53BP1 Santa Cruz sc-22760 Rabbit 1:500 Immunoblotting, immunofluorescence 53BP1 Novus Bio NB100-304 Rabbit 1:500 Immunofluorescence 53BP1 PhosphoT1609/S1618 Antagene Custom made Rabbit 1:100 Immunoblotting, Immunofluorescence Alphatubulin Sigma T6074 Mouse 1:4000 Immunoblotting c-Myc (9E10) Biolegend 658502 Mouse 15ul slurry per mg lysate Immunoprecipitation c-Myc Santa Cruz sc-40 Mouse 1:1000 Immunoblotting c-Myc Santa Cruz sc-789 Rabbit 1:1000 Immunoblotting CDK1 Abcam ab133327 Rabbit 1:1000 Immunoblotting CDK5 Santa Cruz sc-173 Rabbit 1:500 Immunoblotting CDK5 Santa Cruz sc-6247 Mouse 1:500 Immunoblotting CDK5 Santa Cruz sc-249 AC Mouse 15 μl slurry per mg lysate Immunoprecipitation Cyclin A Santa Cruz sc-271682 Mouse 1:100 Immunofluorescence Cyclin B1 Santa Cruz sc-245 Mouse 1:100 Immunoblotting FIBP EpiGentek A50225 Rabbit 1:500 Immunoblotting FLAG Sigma- Aldrich F1804 Mouse 1:1000 Immunoblotting FLAG SigmaAldrich F7425 Rabbit 1:1000 Immunoblotting FLAG (M2) SigmaAldrich A2220 Mouse 15ul slurry per mg lysate Immunoprecipitation KIAA0528 Bethyl A301-469A Rabbit 1:1000 Immunoblotting PhosphoHistone H2AX (Ser139) EMD Millipore 05-636 Mouse 1:500 Immunofluorescence PhosphoHistone H3 (Ser 10) Cell Signaling 9701 Rabbit 1:1000 Immunoblotting PhosphoHistone H3 (Ser 10) AlexaFluor 488 conjugated EMD Millipore 06-570- AF488 Rabbit 1:200 Flow cytometry PP4C Bethyl A300-835A Rabbit 1:1000 Immunoblotting PP4R3β PhosphoS840 GL Biochem Ltd Custom made Rabbit 1:80 Immunoblotting, Immunofluorescence PP4R3β Bethyl A300-842A Rabbit 1:1000 Immunoblotting Protein A/G PLUS agarose Santa Cruz Sc-2003 N/A 20 μl slurry per mg lysate Immunoprecipitation Vinculin Santa Cruz sc-25336 Mouse 1:2000 Immunoblotting Alexa FluorTM 488 anti-mouse IgG Invitrogen A11001 Goat 1:1000 Immunofluorescence Alexa FluorTM 488 anti-rabbit IgG Invitrogen A11034 Goat 1:1000 Immunofluorescence Alexa FluorTM 594 anti-mouse IgG Invitrogen A21203 Donkey 1:1000 Immunofluorescence Alexa FluorTM 594 anti-rabbit IgG Invitrogen A21207 Donkey 1:1000 Immunofluorescence ..
Immunoprecipitation:Article Title: A mitotic CDK5-PP4 phospho-signaling cascade primes 53BP1 for DNA repair in G1
Article Snippet: .. List of antibodies used in this study Target Manufacturer Catalogue number Host Dilution or amount used Application p35/25 Cell Signaling Technology 2680 Rabbit 1:500 Immunoblotting 53BP1 Santa Cruz sc-22760 Rabbit 5ug per mg lysate Immunoprecipitation 53BP1 Santa Cruz sc-22760 Rabbit 1:500 Immunoblotting, immunofluorescence 53BP1 Novus Bio NB100-304 Rabbit 1:500 Immunofluorescence 53BP1 PhosphoT1609/S1618 Antagene Custom made Rabbit 1:100 Immunoblotting, Immunofluorescence Alphatubulin Sigma T6074 Mouse 1:4000 Immunoblotting c-Myc (9E10) Biolegend 658502 Mouse 15ul slurry per mg lysate Immunoprecipitation c-Myc Santa Cruz sc-40 Mouse 1:1000 Immunoblotting c-Myc Santa Cruz sc-789 Rabbit 1:1000 Immunoblotting CDK1 Abcam ab133327 Rabbit 1:1000 Immunoblotting CDK5 Santa Cruz sc-173 Rabbit 1:500 Immunoblotting CDK5 Santa Cruz sc-6247 Mouse 1:500 Immunoblotting CDK5 Santa Cruz sc-249 AC Mouse 15 μl slurry per mg lysate Immunoprecipitation Cyclin A Santa Cruz sc-271682 Mouse 1:100 Immunofluorescence Cyclin B1 Santa Cruz sc-245 Mouse 1:100 Immunoblotting FIBP EpiGentek A50225 Rabbit 1:500 Immunoblotting FLAG Sigma- Aldrich F1804 Mouse 1:1000 Immunoblotting FLAG SigmaAldrich F7425 Rabbit 1:1000 Immunoblotting FLAG (M2) SigmaAldrich A2220 Mouse 15ul slurry per mg lysate Immunoprecipitation KIAA0528 Bethyl A301-469A Rabbit 1:1000 Immunoblotting PhosphoHistone H2AX (Ser139) EMD Millipore 05-636 Mouse 1:500 Immunofluorescence PhosphoHistone H3 (Ser 10) Cell Signaling 9701 Rabbit 1:1000 Immunoblotting PhosphoHistone H3 (Ser 10) AlexaFluor 488 conjugated EMD Millipore 06-570- AF488 Rabbit 1:200 Flow cytometry PP4C Bethyl A300-835A Rabbit 1:1000 Immunoblotting PP4R3β PhosphoS840 GL Biochem Ltd Custom made Rabbit 1:80 Immunoblotting, Immunofluorescence PP4R3β Bethyl A300-842A Rabbit 1:1000 Immunoblotting Protein A/G PLUS agarose Santa Cruz Sc-2003 N/A 20 μl slurry per mg lysate Immunoprecipitation Vinculin Santa Cruz sc-25336 Mouse 1:2000 Immunoblotting Alexa FluorTM 488 anti-mouse IgG Invitrogen A11001 Goat 1:1000 Immunofluorescence Alexa FluorTM 488 anti-rabbit IgG Invitrogen A11034 Goat 1:1000 Immunofluorescence Alexa FluorTM 594 anti-mouse IgG Invitrogen A21203 Donkey 1:1000 Immunofluorescence Alexa FluorTM 594 anti-rabbit IgG Invitrogen A21207 Donkey 1:1000 Immunofluorescence ..
Immunofluorescence:Article Title: A mitotic CDK5-PP4 phospho-signaling cascade primes 53BP1 for DNA repair in G1
Article Snippet: .. List of antibodies used in this study Target Manufacturer Catalogue number Host Dilution or amount used Application p35/25 Cell Signaling Technology 2680 Rabbit 1:500 Immunoblotting 53BP1 Santa Cruz sc-22760 Rabbit 5ug per mg lysate Immunoprecipitation 53BP1 Santa Cruz sc-22760 Rabbit 1:500 Immunoblotting, immunofluorescence 53BP1 Novus Bio NB100-304 Rabbit 1:500 Immunofluorescence 53BP1 PhosphoT1609/S1618 Antagene Custom made Rabbit 1:100 Immunoblotting, Immunofluorescence Alphatubulin Sigma T6074 Mouse 1:4000 Immunoblotting c-Myc (9E10) Biolegend 658502 Mouse 15ul slurry per mg lysate Immunoprecipitation c-Myc Santa Cruz sc-40 Mouse 1:1000 Immunoblotting c-Myc Santa Cruz sc-789 Rabbit 1:1000 Immunoblotting CDK1 Abcam ab133327 Rabbit 1:1000 Immunoblotting CDK5 Santa Cruz sc-173 Rabbit 1:500 Immunoblotting CDK5 Santa Cruz sc-6247 Mouse 1:500 Immunoblotting CDK5 Santa Cruz sc-249 AC Mouse 15 μl slurry per mg lysate Immunoprecipitation Cyclin A Santa Cruz sc-271682 Mouse 1:100 Immunofluorescence Cyclin B1 Santa Cruz sc-245 Mouse 1:100 Immunoblotting FIBP EpiGentek A50225 Rabbit 1:500 Immunoblotting FLAG Sigma- Aldrich F1804 Mouse 1:1000 Immunoblotting FLAG SigmaAldrich F7425 Rabbit 1:1000 Immunoblotting FLAG (M2) SigmaAldrich A2220 Mouse 15ul slurry per mg lysate Immunoprecipitation KIAA0528 Bethyl A301-469A Rabbit 1:1000 Immunoblotting PhosphoHistone H2AX (Ser139) EMD Millipore 05-636 Mouse 1:500 Immunofluorescence PhosphoHistone H3 (Ser 10) Cell Signaling 9701 Rabbit 1:1000 Immunoblotting PhosphoHistone H3 (Ser 10) AlexaFluor 488 conjugated EMD Millipore 06-570- AF488 Rabbit 1:200 Flow cytometry PP4C Bethyl A300-835A Rabbit 1:1000 Immunoblotting PP4R3β PhosphoS840 GL Biochem Ltd Custom made Rabbit 1:80 Immunoblotting, Immunofluorescence PP4R3β Bethyl A300-842A Rabbit 1:1000 Immunoblotting Protein A/G PLUS agarose Santa Cruz Sc-2003 N/A 20 μl slurry per mg lysate Immunoprecipitation Vinculin Santa Cruz sc-25336 Mouse 1:2000 Immunoblotting Alexa FluorTM 488 anti-mouse IgG Invitrogen A11001 Goat 1:1000 Immunofluorescence Alexa FluorTM 488 anti-rabbit IgG Invitrogen A11034 Goat 1:1000 Immunofluorescence Alexa FluorTM 594 anti-mouse IgG Invitrogen A21203 Donkey 1:1000 Immunofluorescence Alexa FluorTM 594 anti-rabbit IgG Invitrogen A21207 Donkey 1:1000 Immunofluorescence ..
Flow Cytometry:Article Title: A mitotic CDK5-PP4 phospho-signaling cascade primes 53BP1 for DNA repair in G1
Article Snippet: .. List of antibodies used in this study Target Manufacturer Catalogue number Host Dilution or amount used Application p35/25 Cell Signaling Technology 2680 Rabbit 1:500 Immunoblotting 53BP1 Santa Cruz sc-22760 Rabbit 5ug per mg lysate Immunoprecipitation 53BP1 Santa Cruz sc-22760 Rabbit 1:500 Immunoblotting, immunofluorescence 53BP1 Novus Bio NB100-304 Rabbit 1:500 Immunofluorescence 53BP1 PhosphoT1609/S1618 Antagene Custom made Rabbit 1:100 Immunoblotting, Immunofluorescence Alphatubulin Sigma T6074 Mouse 1:4000 Immunoblotting c-Myc (9E10) Biolegend 658502 Mouse 15ul slurry per mg lysate Immunoprecipitation c-Myc Santa Cruz sc-40 Mouse 1:1000 Immunoblotting c-Myc Santa Cruz sc-789 Rabbit 1:1000 Immunoblotting CDK1 Abcam ab133327 Rabbit 1:1000 Immunoblotting CDK5 Santa Cruz sc-173 Rabbit 1:500 Immunoblotting CDK5 Santa Cruz sc-6247 Mouse 1:500 Immunoblotting CDK5 Santa Cruz sc-249 AC Mouse 15 μl slurry per mg lysate Immunoprecipitation Cyclin A Santa Cruz sc-271682 Mouse 1:100 Immunofluorescence Cyclin B1 Santa Cruz sc-245 Mouse 1:100 Immunoblotting FIBP EpiGentek A50225 Rabbit 1:500 Immunoblotting FLAG Sigma- Aldrich F1804 Mouse 1:1000 Immunoblotting FLAG SigmaAldrich F7425 Rabbit 1:1000 Immunoblotting FLAG (M2) SigmaAldrich A2220 Mouse 15ul slurry per mg lysate Immunoprecipitation KIAA0528 Bethyl A301-469A Rabbit 1:1000 Immunoblotting PhosphoHistone H2AX (Ser139) EMD Millipore 05-636 Mouse 1:500 Immunofluorescence PhosphoHistone H3 (Ser 10) Cell Signaling 9701 Rabbit 1:1000 Immunoblotting PhosphoHistone H3 (Ser 10) AlexaFluor 488 conjugated EMD Millipore 06-570- AF488 Rabbit 1:200 Flow cytometry PP4C Bethyl A300-835A Rabbit 1:1000 Immunoblotting PP4R3β PhosphoS840 GL Biochem Ltd Custom made Rabbit 1:80 Immunoblotting, Immunofluorescence PP4R3β Bethyl A300-842A Rabbit 1:1000 Immunoblotting Protein A/G PLUS agarose Santa Cruz Sc-2003 N/A 20 μl slurry per mg lysate Immunoprecipitation Vinculin Santa Cruz sc-25336 Mouse 1:2000 Immunoblotting Alexa FluorTM 488 anti-mouse IgG Invitrogen A11001 Goat 1:1000 Immunofluorescence Alexa FluorTM 488 anti-rabbit IgG Invitrogen A11034 Goat 1:1000 Immunofluorescence Alexa FluorTM 594 anti-mouse IgG Invitrogen A21203 Donkey 1:1000 Immunofluorescence Alexa FluorTM 594 anti-rabbit IgG Invitrogen A21207 Donkey 1:1000 Immunofluorescence ..
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